Linda L Pearce

  • Research Assistant Professor
  • Faculty in Environmental and Occupational Health

Our research interests center around the response of the mitochondria and hemoglobin to environmental stressors (i.e. cyanide, sulfide and radiation). Electron paramagnetic resonance (EPR), done in conjunction with Prof. Mike Hendrich at CMU, also provides a useful probe for our investigations.


University of South Florida, BS Clinical Chemistry
Iowa State University, PhD Inorganic Chemistry


Environmental Health Chemistry (EOH 2309)

Selected Publications

J. Kanai, L.L. Pearce, P.R. Clemens, L.A. Birder, M.M. VanBibber, S.-Y. Choi, W.C. deGroat, J. Peterson: “Identification of a Neuronal Nitric Oxide Synthase in Isolated Cardiac Mitochondria Using Electrochemical Detection”; Proc. Natl. Acad. Sci. USA, 2001, 98, 14126-14131.

L.L. Pearce, A.J. Kanai, L.A. Birder, B.R. Pitt, J. Peterson: “The Catabolic Fate of Nitric
Oxide: The Nitric Oxide Oxidase and Peroxynitrite Reductase Activities of Cytochrome Oxidase”; J. Biol. Chem., 2002, 277, 13556-13562.

L.L. Pearce*, E.L. Bominaar, J. Peterson*: “VisibleRegion MCD and MLD Spectra of Nitrosylferrohemoglobin and Oxyhemoglobin”; Biochem. Biophys. Res. Comm., 2002, 297, 220-223.

A. Fago, A.L. Crumbliss, J. Peterson, L.L. Pearce, C. Bonaventura*: “The Case of the Missing NO-hemoglobin: Spectral Changes Suggestive of Heme Redox Reactions Reflect Changes in NO-heme Geometry”; Proc. Natl. Acad. Sci. USA 2003, 100, 12087-12092.

L.L. Pearce*, E.L. Bominaar, B.C. Hill, J. Peterson*: “Reversal of Cyanide Inhibition of Cytochrome c Oxidase by the Auxiliary Substrate Nitric Oxide: An Endogenous Antidote to Cyanide Poisoning?”; J. Biol. Chem. 2003, 278, 52139-52145.

A. Kanai*, M. Epperly, L. Pearce, L. Birder, M. Zeidel, S. Meyers, J. Greenberger, W. de
Groat, G. Apodaca, J. Peterson: “Differing Roles of Mitochondrial Nitric Oxide Synthase in Cardiomyocytes and Urothelial Cells”; Am. J. Physiol. Heart Circ. Physiol.2004, 286, H13-H21.

J. Peterson*, A.J. Kanai, L.L. Pearce: “A Mitochondrial Role for Catabolism of Nitric Oxide in Cardiomyocytes not Involving Oxymyoglobin”; Am. J. Physiol. Heart Circ. Physiol. 2004, 286, H55-H58.

T. Chen, L.L. Pearce, J. Peterson, D. Stoyanovsky, T.R. Billiar*: “Glutathione Depletion Renders Rat Hepatocytes Sensitive to Nitric Oxide-donor Mediated Toxicity”; Hepatology 2005, 42, 598-607.

Pearce, L.L.; Kanai, A.J.; Epperly, M.W.; Peterson, J., “Nitrosative Stress Results in&nbsp Irreversible Inhibition of Purified Mitochondrial Complexes I and II without Modification of Cofactors,” Nitric Oxide 2005 13, 254-263.

L. L. Pearce, E. L. Manzano, S. Martinez-Bosch and J. Peterson: “Antagonism of Nitric Oxide Toward the Inhibition of Cytochrome c oxidase by Carbon Monoxide and Cyanide”; Chem. Res. Toxicol. 21(11), 23073-2081, 2008.

L. L. Pearce, S. Martinez-Bosch, E. L. Manzano, D. E. Winnica, M. W. Epperly and
J. Peterson: “The Resistance of Electron Transport Chain Fe-S Clusters to
Oxidative Damage during the Reaction of Peroxynitrite with Mitochondrial
Complex II and Rat Heart Pericardium”; Nitric Oxide 20 (2009)135-142, doi: 10.1016/j.niox.2008.12.001.

M. W. Epperly, J. A. Melendez, X. Zhang, S. Nie, L. L. Pearce, J. Peterson, D. Franicola, T. Dixon, B. A. Greenberger, P. Komanduri, H. Wong and J. S.Greenberger: “Mitochondrial Targeting of a Catalase Transgene Product by Plasmid Liposomes Increases Radioresistance In Vitro and In Vivo”; Radiation Research (2009) 171, 588-595.

Molly Stitt-Fischer, Rachel K. Ungerman, Daniel S. Wilen, Lara M. Huyler, Shannon E.
Raub, Jim Peterson and Linda L. Pearce. “Manganese Superoxide Dismutase is not
Radioprotective in Bovine Pulmonary Artery Endothelial Cells at Systemic Oxygen
Levels”: Radiation Research, (2010) 174, 679–690.

Mai Otsuka, Sarah A. Marks, Daniel E. Winnica, Andrew A. Amoscato, Linda L. Pearce and Jim Peterson “Covalent Modifications of Hemoglobin by Nitrite Anion: Formation Kinetics and Properties of Nitrihemoglobin”; Chemical Research in Toxicology. (2010), 23, 1786–1795.

Leah K. Cambal, Megan R. Swanson, Quan Yuan, Andrew C. Weitz, Hui-Hua Li, Bruce R. Pitt, Linda L. Pearce and Jim Peterson “Acute, Sub-lethal Cyanide Poisoning in Mice is Ameliorated by Nitrite Alone: Complications Arising from Concomitant Administration of Nitrite and Thiosulfate as an Antidotal Combination”; Chemical Research in Toxicology, (2011) 24, 1104–1112 published in the July issue as a featured article (cover).

Oscar S. Benz, Quan Yuan, Andrew A. Amoscato, Linda L. Pearce, Jim Peterson “Co(III)TMPyP is an Antidote for Acute Cyanide Toxicity” Chemical Research in Toxicology. (2012) 25, 2687–2703.

Sang-Min LeeJoseph N. McLaughlin, Daniel R. Frederick1, Lin Zhu, Karla Wasserloos, Iris Kaminski2, Octavia Peck-Palmer, Linda Pearce, Jim Peterson, CBI Person, Jin; Joseph D. Latoche, Tim D. Oury, Cheryl L. Fattman, Donna Beer-Stolz3, John F. Alcorn4, Derek C. Angus, Bruce R. Pitt, A. Murat Kaynar&nbsp “Metallothionien-induced zincpartitioning exacerbates hyperoxic induced lung injury”, Am J PhysiolLung Cell Mol Physiol March 1, (2013) 304:L350-L360

Leah K. Cambal Andrew C. Weitz, Hui-Hua Li, Xi Zheng, Linda L. Pearce and Jim Peterson “Alternative Nitrite Therapies for Acute Cyanide Poisoning in Mice: Complications Arising from Anesthetics” Chemical Research in Toxicology. (2013) May 20;26(5):828-36. PMID:23536974.

Angela Fago?§, Alvin L. Crumbliss, Michael P. Hendrich?, Linda L. Pearce**, Jim Peterson**, Robert Henkens§, and Celia Bonaventura&sect “Oxygen Binding to Partially Nitrosylated Hemoglobin” Biochimica Biophysica Acta - Proteins and Proteomics Biochimica et Biophysica Acta. (2013) April 25. PMID:23624264.

Samantha L Malone, Linda L Pearce & Jim Peterson. Environmental Toxicology of Cyanide. Clinical and Experimental Toxicology of Cyanide. Chichester, UK: John Wiley & Sons. 2015.

Andrea A. Cronican, Kristin L. Frawley, Humza Ahmed, Linda L. Pearce* and Jim Peterson* “Antagonism of Acute Sulfide Poisoning in Mice by Nitrite Anion without Methemoglobinemia&rdquo Chemical Research in Toxicology (2015) 28(7) 1398-1408.

Oscar S. Benz, Quan Yuan, Andrea A. Cronican, Jim Peterson* and Linda L. Pearce* "Effect of Ascorbate on the Cyanide-Scavenging Capability of Cobalt(III) meso-Tetra(4-N-methylpyridyl)porphine Pentaiodide: Deactivation by Reduction?” Chemical Research in Toxicology (2016) 29(3) 270-278.

Elisenda Lopez-Monzano, Andrea A. Cronican, Kristin L. Frawley, Jim Peterson* and Linda L. Pearce* “Cyanide Scavenging by a Cobalt Schiff-base Macrocycle: A Cost-effective Alternative to Corrinoids” Chemical Research in Toxicology (2016) April 22, DOI: 10.1021/acs.chemrestox.6b00070

Samantha L. Malone Rubright, Linda L. Pearce* and Jim Peterson** “Environmental Toxicology of Hydrogen Sulfide” (invited review) Nitric Oxide (2017) 71 (1) 1-13. DOI: 10.1016/j.niox.2017.09.011

Quan Yuan, Linda L. Pearce,* and Jim Peterson* “Relative Propensities of Cytochrome c Oxidase and Cobalt Corrins for Reaction with Cyanide and Oxygen: Implications for Amelioration of Cyanide Toxicity” Chemical Research in Toxicology (2017) 30(12) 2197-2308. DOI: 10.1021/acs.chemrestox.7b00275&nbsp

Kristin L. Frawley, Andrea A. Cronican, Linda L. Pearce,* and Jim Peterson* “Sulfide Toxicity and Its Modulation by Nitric Oxide in Bovine Pulmonary Artery Endothelial Cells” Chemical Research in Toxicology (2017) 30(12) 2100-2009. DOI: 10.1021/acs.chemrestox.7b00147

Andrea A. Cronican, Kristin L. Frawley, Erin P. Straw, Elisenda Lopez-Manzano, Hirunwut Praekunatham, Jim Peterson,* and Linda L. Pearce* “A Comparison of the Cyanide-Scavenging Capabilities of Some Cobalt-Containing Complexes in Mice” Chemical Research in Toxicology (2018) 31(4) 259-268. DOI: 10.1021/acs.chemrestox.7b00314

Kristin L. Frawley, Hirunwut Praekunatham, Andrea A. Cronican, Jim Peterson* and Linda L. Pearce* “Assessing Modulators of Cytochrome cOxidase Activity in Galleria mellonella Larvae”Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology (2019) 219

Kimberly K. Garrett, Kristin L. Frawley, Samantha Carpenter Totoni, Yookyung Bae, Jim Peterson* and Linda L. Pearce* “The Antidotal Action of Some Gold(I) Complexes Toward Phosphine Toxicity”Chemical Research in Toxicology (2019) May 16. doi:10.1021/acs.chemrestox.9b00095. [Epub ahead of print]

Hirunwut Praekunatham, Linda L. Pearce* and Jim Peterson* “Reaction kinetics of cyanide binding to a cobalt Schiff-base macrocycle relevant to its mechanism of antidotal action”Chemical Research in Toxicology(2019)32 1630-1637 DOI 10.1021/acs.chemrestox.9b00170..

Hirunwut Praekunatham, Kimberly K. Garrett, Yookyung Bae, Andrea A. Cronican, Kristin L. Frawley, Linda L. Pearce* and Jim Peterson* “A Cobalt Schiff-base Complex as a Putative Therapeutic for Azide Poisoning”Chemical Research in Toxicology(2019)33 333-342 DOI 10.1021/acs.chemrestox.9b00229.

Kristin L. Frawley, Samantha Carpenter Totoni, Yookyung Bae, Linda L. Pearce,* and Jim Peterson* “A Comparison of Potential Azide Antidotes in a Mouse Model”Chemical Research in Toxicology(2019)33 594-603 DOI 10.1021/acs.chemrestox.9b00422.

Anna C. Zemke, Cody J. Madison, N. Kasturiarachi, L.L. Pearce and Jim Peterson “Antimicrobial Synergism toward Psedomonas aeruginosa by Gallium(III) and Inorganic Nitrite”Frontiers in Microbiology vol 11, 1-8 (2020)DOI:10.3389/fmicb.2020.0211